Phosphorylation of Guanosine Using Guanosine-inosine Kinase from Exiguobacterium acetylicum Coupled with ATP Regeneration.

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  • KAWASAKI Hisashi
    Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc.
  • USUDA Yoshihiro
    Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc.
  • SHIMAOKA Megumi
    Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc.
  • UTAGAWA Takashi
    Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc.

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  • Phosphorylation of Guanosine Using Guanosine-inosine Kinase from<i>Exiguobacterium acetylicum</i>Coupled with ATP Regeneration

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Guanosine 5′-monophosphate (5′-GMP) and inosine 5′-monophosphate (5′-IMP) are widely used as flavor enhancers. Recently, a novel process for 5′-IMP production by phosphorylation of inosine using guanosine-inosine kinase coupled with ATP regeneration was reported.1) In this study, we demonstrated the practical possibility of producing 5′-GMP by phosphorylation of guanosine using a guanosine-inosine kinase from Exiguobacterium acetylicum coupled with ATP regeneration.<br>

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