In Vitro and in Vivo Anti-platelet Effects of Enzymatic Hydrolysates of Collagen and Collagen-related Peptides.

NONAKA Isao, KATSUDA Shin-ichiro, OHMORI Takashi, SHIGEHISA Tamotsu, NAKAGAMI Tatsuyoshi, MARUYAMA Susumu

doi:10.1271/bbb.61.772

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In Vitro and in Vivo Anti-platelet Effe
<i>In Vitro</i> and <i>in Vivo</i> Anti-platelet Effects of Enzymatic Hydrolysates of Collagen and Collagen-related Peptides

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Collagen-related peptides, Gly-Pro-Arg and its analogues, were examined for their inhibitory effects on platelet aggregation induced by the addition of ADP. Human platelet aggregation was suppressed by more than 50% with each of Gly-Pro-Arg and such Gly-Pro-Arg-containing peptides as Gly-Pro-Arg-Gly, Gly-Pro-Arg-Gly-Pro, Gly-Pro-Arg-Pro-Pro, and Gly-Pro-Arg-Pro-Pro-Pro at a concentration of 0.3 mM. The inhibitory effects of these peptides were about 10 times higher in human PRP than in rat PRP. Other Gly-Pro-Arg analogues such as Sar-Pro-Arg, Gly-Pro-Lys, Gly-Ala-Arg, and Ala-Gly-Pro-Arg had no inhibitory effect at a concentration from 0.1 to 0.8 mM even in human PRP. Intravenous and oral administrations of Gly-Pro-Arg and enzymatic hydrolysates of collagen suppressed the decrease in platelet count for endotoxin-induced DIC in rats. Collagen itself has been regarded as a potent inducer of platelet aggregation, but these findings suggest that collagen-related peptides and enzymatic hydrolysates of collagen prevent platelet aggregation.

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Bioscience, Biotechnology, and Biochemistry

Bioscience, Biotechnology, and Biochemistry 61 (5), 772-775, 1997

Japan Society for Bioscience, Biotechnology, and Agrochemistry

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