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Pectin Methylesterase Gene (pmeA) from Aspergillus oryzae KBN616: Its Sequence Analysis and Overexpression, and Characterization of the Gene Product.
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- KITAMOTO Noriyuki
- Food Research Institute, Aichi Prefectural Government
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- OKADA Hideki
- Shin Nihon Chemical Co. Ltd.
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- YOSHINO Shoko
- Food Research Institute, Aichi Prefectural Government
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- OHMIYA Kunio
- Faculty of Bioresources, Mie University
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- TSUKAGOSHI Norihiro
- Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
Bibliographic Information
- Other Title
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- Pectin Methylesterase Gene (<i>pmeA</i>) from<i>Aspergillus oryzae</i>KBN616: Its Sequence Analysis and Overexpression, and Characterization of the Gene Product
- Pectin methylesterase gene (pmeA) from Aspergillus oryzae KBN616: its sequence analsyis and overexpression, and characterization of the gene product
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Description
A gene (pmeA) encoding pectin methylesterase was isolated from a shoyu koji mold, Aspergillus oryzae KBN616, and characterized. The structural gene comprised 1,370 bp with six introns. The PMEA protein consisted of 331 amino acids with a putative signal peptide of 17 amino acids. The deduced amino acid sequence was very similar to those of Aspergillus niger PMEA and Aspergillus aculeatus PME1. The pmeA gene was efficiently expressed under control of the A. oryzae TEF1 gene promoter for purification and characterization of the ezymatic properties. PMEA had a molecular mass of 38.5 kDa, a pH optimum of 5.0, and a temperature optimum of 55°C.<br>
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 63 (1), 120-124, 1999
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681451115648
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- NII Article ID
- 110002679351
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1MXhtVCkur8%3D
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- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
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- NDL BIB ID
- 4653720
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- PubMed
- 10052131
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- JaLC
- NDL Search
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed