Purification and Characterization of Glycoprotein from the Skin Mucus of the Rainbow Trout, Salmo gairdneri.

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  • Purification and Characterization of Gl
  • Purification and Characterization of Glycoprotein from the Skin Mucus of the Rainbow Trout,<i>Salmo gaivdneri</i>

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Abstract

Mucus glycoprotein (RGP) was purified and characterized from the skin mucus of rainbow trout, Salmo gairdneri. RGP was found to contain 30.1% NeuAc, 26.0% GalNAc, 5.0% Gal, and 26.0% amino acids. The protein moiety of RGP is very rich in Thr (32.4 mol%). Neither NeuGc nor KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid) was found in RGP. Alkaline borohydride treatment of RGP yielded a major disaccharide alditol, NeuAcx2→6GalNAc-ol and more than 4 minor oligosaccharide alditols including NeuAc→(GalNAcx1→)GalNAc-ol. It was evident that an average RGP molecule has approximately 500 NeuAc-containing oligosaccharide chains, which are attached to the Thr and Ser residues of the protein moiety and spaced at an average of 3 amino acids apart.

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