Isolation and Molecular Characterization of the Locked-on Mutant of Mg〔2+〕 Sensor PhoQ in Escherichia coli
-
- MINAGAWA Shu
- Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University
-
- OKURA Ryouta
- Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University
-
- TSUCHITANI Hiroki
- Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University
-
- HIRAO Kiyo
- Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University
-
- YAMAMOTO Kaneyoshi
- Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University
-
- UTSUMI Ryutaro
- Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University
書誌事項
- タイトル別名
-
- Isolation and Molecular Characterization of the Locked-on Mutant of Mg2+ Sensor PhoQ in Escherichia coli
- Isolation and Molecular Characterization of the Locked-on Mutant of Mg<sup>2+</sup>Sensor PhoQ in<i>Escherichia coli</i>
この論文をさがす
説明
A Mg2+ sensor mutant (PhoQD179L(A)) in which D179 of PhoQ was changed into L or A was isolated and characterized in Escherichia coli. PhoQ–PhoP regulon genes, phoPQ, mgtA and mgrB transcriptions were repressed at a high Mg2+ concentration in WQ3007 (phoQ-defective strain)/pHO119, but not in WQ3007/pHO179L(A). The in vitro autophosphorylation activity of membrane-bound PhoQ was repressed by Mg2+ (10 mM), but that of membrane-bound PhoQD179L(A) was not. Furthermore, the phosphotransfer from membrane-bound PhoQ to PhoP was also repressed by Mg2+, but was not observed in membrane-bound PhoQD179L(A). These results suggest that PhoQD179L(A) is a locked-on mutant that is defective in extracellular Mg2+-sensing and that the D179 amino acid residue of PhoQ plays an essential role in signal transfer between the Mg2+-sensory and histidine kinase domain of PhoQ.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 69 (7), 1281-1287, 2005
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681451195520
-
- NII論文ID
- 130000030268
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 7378343
-
- PubMed
- 16041131
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- JaLC
- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
-
- 抄録ライセンスフラグ
- 使用不可