Molecular Cloning of a Novel Gene,<i>dtsR</i>, Which Rescues the Detergent Sensitivity of a Mutant Derived from<i>Brevibacterium lactofermentum</i>

DOI Web Site PubMed 被引用文献13件 参考文献41件

書誌事項

タイトル別名
  • Molecular Cloning of a Novel Gene, dtsR, Which Rescues the Detergent Sensitivity of a Mutant Derived from Brevibacterium lactofermentum.
  • Molecular cloning of a novel gene dtsR, which rescues detergent sensitivity of a mutant derived from Brevibacterium lactofermentum

この論文をさがす

説明

Several strains of Corynebacterium and Brevibacterium are known for their ability to secrete large amounts of amino acids, especially L-glutamate. We focused on the mechanism of L-glutamate secretion triggered by a detergent, namely polyoxyethylenesorbitan monopalmitate (PESP). A mutant strain, AJ11060, derived from Brevibacterium lactofermentum ATCC 13869 indicates the sensitivity to PESP. A multicopy suppresser gene that compliments the sensitivity of AJ11060 to the detergent was derived from a gene library of B. lactofermentum AJ12036. A 2855-bp DNA fragment was cloned and sequenced. An open reading frame was found that coded for the rescuer gene of the sensitivity to PESP of AJ11060 and was designated dtsR. The expression of the dtsR gene in B. lactofermentum was confirmed by using anti-DtsR antibody. The deduced DtsR protein indicated significant homology with some biotin enzymes such as the β chain of propionyl-CoA carboxylase from rat (48.3%) and human (48.7%), or a 12S chain of methylmalonyl-CoA carboxyltransferase from Propionibacterium freudenreichii (43.1%).

収録刊行物

被引用文献 (13)*注記

もっと見る

参考文献 (41)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ