Enzymatic Quantification of<scp>L</scp>-Tartrate in Wines and Grapes by Using the Secondary Activity of<scp>D</scp>-Malate Dehydrogenase
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- TSUKATANI Tadayuki
- Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
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- MATSUMOTO Kiyoshi
- Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
書誌事項
- タイトル別名
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- Enzymatic Quantification of L-Tartrate in Wines and Grapes by Using the Secondary Activity of D-Malate Dehydrogenase.
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抄録
L-Tartrate in wines and grapes was enzymatically quantified by using the secondary activity of D-malate dehydrogenase (D-MDH). NADH formed by the D-MDH reaction was monitored spectrophotometrically. Under the optimal conditions, L-tartrate (a 1.0 mM sample solution) was fully oxidized by D-MDH in 30 min. A linear relationship was obtained between the absorbance difference and the L-tartrate concentration in the range of a 0.02-1.0 mM sample solution with a correlation coefficient of 0.9991. The relative standard deviation from ten measurements was 1.71% at the 1.0 mM sample solution level. The proposed method was compared with HPLC, and the values determined by both methods were in good agreement.<br>
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 63 (10), 1730-1735, 1999
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681451543552
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- NII論文ID
- 110002691993
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1MXnt1Gjsbc%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4899215
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
