Differential Scanning Calorimetry of the Effects of Ca2+ on the Thermal Unfolding of Pseudomonas cepacia Lipase.
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- TANAKA Akiyoshi
- <i>Faculty of Bioresources, Mie University</i>
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- SUGIMOTO Hayuki
- <i>Faculty of Bioresources, Mie University</i>
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- MUTA Yuko
- <i>Graduate School of Agriculture, Kyoto University</i>
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- MIZUNO Takafumi
- <i>Faculty of Bioresources, Mie University</i>
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- SENOO Keishi
- <i>Graduate School of Agricultural and Life Sciences, The University of Tokyo</i>
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- OBATA Hitoshi
- <i>Faculty of Bioresources, Mie University</i>
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- INOUYE Kuniyo
- <i>Graduate School of Agriculture, Kyoto University</i>
Bibliographic Information
- Other Title
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- Differential Scanning Calorimetry of the Effects of Ca〔2+〕 on the Thermal Unfolding of Pseudomonas cepacia Lipase
- Differential Scanning Calorimetry of the Effects of Ca<sup>2+</sup>on the Thermal Unfolding of<i>Pseudomonas cepacia</i>Lipase
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Abstract
Thermal unfolding of P. cepacia lipase was observed by adiabatic differential scanning microcalorimetry in the absence and presence of calcium ions at pH 8, and thermodynamic parameters of unfolding were evaluated to analyze the unfolding mechanism of the enzyme. The temperature of unfolding was higher at higher concentrations of Ca2+. From the Ca2+ concentration-dependence of the unfolding temperature, the number of calcium ions that dissociated from the enzyme molecule upon unfolding was estimated to be one. These results confirmed the validity of the unfolding mechanism proposed previously: NCa2+↔D+Ca2+, where N and D represent the native and denatured states, respectively, of the enzyme.<br>
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 67 (1), 207-210, 2003
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681451583872
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- NII Article ID
- 110002693974
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3sXhtVWqtbk%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 6423570
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- PubMed
- 12619699
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed