Characterization of Partially Truncated Human Midkine Expressed in<i>Pichia pastoris</i>

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  • Characterization of Partially Truncated Human Midkine Expressed in Pichia pastoris.

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  Recombinant human midkine (rh-midkine) was expressed under the control of the AOX1 gene promoter in Pichia pastoris. Approximately 640 mg of rh-midkine was secreted into one liter of medium of the high cell-density fermentation. The protein processing of the rh-midkine was done efficiently and correctly in P. pastoris, and O-mannosylation was not detected in the purified rh-midkine. However, only about 30% of the purified rh-midkine was intact. The other ones lost 5-12 amino acid residues from the amino-termini, provably by proteolysis. Even the mixture of these truncated midkines could promote CHO cell proliferation as well as the authentic rh-midkine.<br>

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