Production of Lupin Acid Phosphatase in Transgenic Rice for Use as a Phytate-hydrolyzing Enzyme in Animal Feed
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- HAMADA Akira
- Functional Chemicals Laboratory, Mitsui Chemicals, Inc.
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- YAMAGUCHI Ken-ichi
- Functional Chemicals Laboratory, Mitsui Chemicals, Inc.
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- HARADA Michiko
- Functional Chemicals Laboratory, Mitsui Chemicals, Inc.
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- NIKUMARU Seiya
- Functional Chemicals Laboratory, Mitsui Chemicals, Inc.
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- WASAKI Jun
- Creative Research Initiative ‘Sousei’ (CRIS), Hokkaido University
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- SHINANO Takuro
- Creative Research Initiative ‘Sousei’ (CRIS), Hokkaido University
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- MATSUI Hirokazu
- Graduate School of Agriculture, Hokkaido University
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- OSAKI Mitsuru
- Graduate School of Agriculture, Hokkaido University
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- HONDA Hideo
- Functional Chemicals Laboratory, Mitsui Chemicals, Inc.
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Abstract
The acid phosphatase gene from lupin was expressed in transgenic rice plants under the control of the maize ubiquitin promoter or rice chlorophyll a/b binding protein (Cab) promoter. Transgenic rice leaves exhibited up to an 18-fold increase in phytate-hydrolyzing activity. Based on the phytate-hydrolyzing activity at pH 5.5, more than 85% this activity was retained after heat-treatment at 80 °C for 15 min, and the heterologous enzyme in leaf sections and leaf extracts was relatively stable during storage. A distinct increase in released phosphate was observed when the heterologous enzyme was mixed with the feed extract. These results suggest that the heterologous enzyme in rice plants may maintain its desired characteristics as a phytate-hydrolyzing enzyme when added to animal feed.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 68 (7), 1611-1616, 2004
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681451798912
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- NII Article ID
- 10013520375
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 7031171
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- PubMed
- 15277775
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed