Production and Characterization of Biosurfactants from<i>Bacillus licheniformis</i>F2.2

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  • THANIYAVARN Jiraporn
    <i>Department of Microbiology, Faculty of Science, Chulalongkorn University</i> <i>Department of Material and Life Science, Graduate School of Engineering, Osaka University</i> <i>Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University</i>
  • ROONGSAWANG Niran
    <i>Department of Material and Life Science, Graduate School of Engineering, Osaka University</i>
  • KAMEYAMA Takayuki
    <i>Department of Material and Life Science, Graduate School of Engineering, Osaka University</i>
  • HARUKI Mitsuru
    <i>Department of Material and Life Science, Graduate School of Engineering, Osaka University</i>
  • IMANAKA Tadayuki
    <i>Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University</i>
  • MORIKAWA Masaaki
    <i>Department of Material and Life Science, Graduate School of Engineering, Osaka University</i>
  • KANAYA Shigenori
    <i>Department of Material and Life Science, Graduate School of Engineering, Osaka University</i>

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  • Production and Characterization of Biosurfactants from Bacillus licheniformis F2.2

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A biosurfactant-producing strain, Bacillus licheniformis F2.2, was isolated from a fermented food in Thailand. The strain was capable of producing a new biosurfactant, BL1193, as well as two kinds of popular lipopeptide biosurfactants, plipastatin and surfactin. Mass spectrometry and FT-IR analysis indicated that BL1193 had a molecular mass of 1,193 Da with no peptide portion in the molecule. While plipastatin and surfactin were abundantly produced in a nutrient YPD medium, BL1193 was produced only in a synthetic DF medium containing no amino acids. According to an oil displacement activity test, the specific activity of BL1193 (6.53 kBS units/mg) is equivalent to that of surfactin (5.78–6.83 kBS units/mg).<br>

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