Med, a Cell-surface Localized Protein Regulating a Competence Transcription Factor Gene, comK, in Bacillus subtilis

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  • OGURA Mitsuo
    <i>Department of Marine Science and Technology, Tokai University</i>
  • HASHIMOTO Hitoshi
    <i>Department of Marine Science and Technology, Tokai University</i>
  • TANAKA Teruo
    <i>Department of Marine Science and Technology, Tokai University</i>

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タイトル別名
  • Med, a Cell-surface Localized Protein Regulating a Competence Transcription Factor Gene, comK, in Bacillus subtlis.
  • Med, a Cell-surface Localized Protein Regulating a Competence Transcription Factor Gene,<i>comK</i>, in<i>Bacillus subtilis</i>

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Med was found as a positive regulator for comK, a master regulator for late competence genes. It was found by Western analysis that the ComK level was decreased in a med mutant. Experiments using an alkaline phosphatase fusion with Med and Western analysis of Med were done because a putative lipo-modification signal is found at the N-terminus of Med. The results obtained are consistent with the localization of Med at the cell surface. An implication of the cell-surface localization of Med is discussed in terms of comK regulation.<br>

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