Structure and Function of Bacterial Super-Biosystem Responsible for Import and Depolymerization of Macromolecules
-
- HASHIMOTO Wataru
- Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University
-
- MOMMA Keiko
- Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University
-
- MARUYAMA Yukie
- Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University
-
- YAMASAKI Masayuki
- Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University
-
- MIKAMI Bunzo
- Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University
-
- MURATA Kousaku
- Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University
Search this article
Abstract
Generally, when microbes assimilate macromolecules, they incorporate low-molecular-weight products derived from macromolecules through the actions of extracellular degrading enzymes. However, a Gram-negative bacterium, Sphingomonas sp. A1, has a smart biosystem for the import and depolymerization of macromolecules. The bacterial cells directly incorporate a macromolecule, alginate, into the cytoplasm through a “superchannel”, as we named it. The superchannel consists of a pit on the cell surface, alginate-binding proteins in the periplasm, and an ATP-binding cassette transporter in the inner membrane. Cytoplasmic polysaccharide lyases depolymerize alginate into the constituent monosaccharides. Other than the proteins characterized so far, novel proteins (e.g., flagellin homologs) have been found to be crucial for the import and depolymerization of alginate through genomics- and proteomics-based identification, thus indicating that the biosystem is precisely constructed and regulated by diverse proteins. In this review, we focus on the structure and function of the bacterial biosystem together with the evolution of related proteins.
Journal
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 69 (4), 673-692, 2005
Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Tweet
Details 詳細情報について
-
- CRID
- 1390282681451978240
-
- NII Article ID
- 130000030348
-
- NII Book ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL BIB ID
- 7308259
-
- PubMed
- 15849405
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed
