Heterogeneous Nuclear Ribonucleoprotein K Interacts with and Is Proteolyzed by Calpain<i>in vivo</i>
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- KIMURA Eiichi
- <i>Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo</i>
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- ABE Keiko
- <i>Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo</i>
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- SUZUKI Koichi
- <i>Tokyo Metropolitan Institute of Gerontology</i>
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- SORIMACHI Hiroyuki
- <i>Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo</i> <i>CREST, JST</i>
書誌事項
- タイトル別名
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- Heterogeneous Nuclear Ribonucleoprotein K Interacts with and Is Proteolyzed by Calpain in vivo
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説明
Calpain is a cytosolic “modulator protease” that modulates cellular functions in response to Ca2+. To identify in vivo substrates of calpain, yeast two-hybrid screening was done using the 5-EF-hand (penta-EF-hand; PEF) domain of the μ-calpain large subunit (domain IV), since several possible in vivo substrates for calpain have been previously reported to bind to the 5-EF-hand domains. Other than the regulatory subunit of calpain, which binds to the domain IV, heterogeneous nuclear ribonucleoproteins (hnRNP) K and R were identified, and shown to be proteolyzed by μ-calpain in vitro. When expressed in COS7 cells, hnRNP K and μ-calpain co-localized in the cytosol, and Ca2+-ionophore stimulation of the cells resulted in proteolysis of hnRNP K, indicating that hnRNP K is an in vivo substrate for calpain. Now, hnRNP K is considered to function as a scaffold protein for its binding proteins, such as PKCδ and C/EBPβ, which were reported to be calpain substrates, suggesting that hnRNP-K is a scaffold for calpain to proteolyze these proteins.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 67 (8), 1786-1796, 2003
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681452151808
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- NII論文ID
- 110002694302
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3sXnt1Wmsrg%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 6665729
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- PubMed
- 12951515
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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