Isolation and Culture of Bovine Mammary Epithelial Cells and Establishment of Gene Transfection Conditions in the Cells.

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Bovine mammary epithelial cells (BMEC) were isolated as acinous fragments from a mammary gland of a lactating cow. They grew well on plastic substratum, showed the characteristic cobblestone morphology of epithelial cells, and secreted αs1-, β-, and k-caseins even when grown on plastic substratum. A plasmid containing the bacterial chloramphenicol acetyl transferase (CAT) gene was transfected to the isolated BMEC by calcium phosphate precipitation and electroporation methods. The transfection efficiency of BMEC by the calcium phosphate method was greatly improved by post-transfection osmotic shock with glycerol or polyethylene glycol. An about 700 bp DNA fragment containing 5'-flanking sequence of bovine αs1-casein gene showed promoter activity in the transfected BMEC. The primary culture of BMEC might be useful for studies on regulation of bovine milk-protein gene expression.

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詳細情報 詳細情報について

  • CRID
    1390282681452176640
  • NII論文ID
    110002677369
  • NII書誌ID
    AA10824164
  • DOI
    10.1271/bbb.59.59
  • COI
    1:CAS:528:DyaK2MXjslKrt74%3D
  • ISSN
    13476947
    09168451
    http://id.crossref.org/issn/09168451
  • PubMed
    7765977
  • Web Site
    http://www.tandfonline.com/doi/pdf/10.1271/bbb.59.59
  • 本文言語コード
    en
  • 資料種別
    journal article
  • データソース種別
    • JaLC
    • Crossref
    • PubMed
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

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