Insertion of Unmarked DNA Sequences in Multiple Loci of the Bacillus subtilis 168 Genome: an Efficient Selection Method
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- UOTSU-TOMITA Rie
- Mitsubishi Kagaku Institute of Life Sciences
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- KANEKO Shinya
- Mitsubishi Kagaku Institute of Life Sciences
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- TSUGE Kenji
- Mitsubishi Kagaku Institute of Life Sciences
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- ITAYA Mitsuhiro
- Mitsubishi Kagaku Institute of Life Sciences
Bibliographic Information
- Other Title
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- Insertion of Unmarked DNA Sequences in Multiple Loci of the<i>Bacillus subtilis</i>168 Genome: an Efficient Selection Method
- Insertion of unmarked sequences in multiple loci of the Bacillus subtilis 168 genome: an efficient selection method
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Abstract
The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 69 (5), 1036-1039, 2005
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681452183296
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- NII Article ID
- 130000030340
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 7322782
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- PubMed
- 15914929
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed