Identification of<i>sn</i>-Glycerol-1-phosphate Dehydrogenase Activity from Genomic Information on a Hyperthermophilic Archaeon,<i>Sulfolobus tokodaii</i>Strain 7

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  • KOGA Yosuke
    Department of Chemistry, School of Medicine, University of Occupational and Environmental Health
  • OHGA Mami
    Department of Chemistry, School of Medicine, University of Occupational and Environmental Health
  • TSUJIMURA Masanari
    National Institute of Advanced Industrial Science and Technology
  • MORII Hiroyuki
    Department of Chemistry, School of Medicine, University of Occupational and Environmental Health
  • KAWARABAYASI Yutaka
    National Institute of Advanced Industrial Science and Technology

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  • Identification of sn-Glycerol-1-phosphate Dehydrogenase Activity from Genomic Information on a Hyperthermophilic Archaeon, Sulfolobus tokodaii Strain 7

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sn-Glycerol-1-phosphate dehydrogenase is responsible for the formation of sn-glycerol-1-phosphate, the backbone of membrane phospholipids of Archaea. This activity had never been detected in cell-free extract of Sulfolobus sp. Here we report the detection of this activity on the thermostable ST0344 protein of Sulfolobus tokodaii expressed in Escherichia coli, which was predicted from genomic information on S. tokodaii. This is another line of evidence for the general mechanism of sn-glycerol-1-phosphate formation by the enzyme.

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