Gene Cloning and Overexpression of a Geranylgeranyl Diphosphate Synthase of an Extremely Thermophilic Bacterium, Thermus themophilus
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- OHTO Chikara
- Bio Research Laboratory, Toyota Motor Corporation Department of Biochemistry and Engineering, Tohoku University
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- ISHIDA Chika
- Bio Research Laboratory, Toyota Motor Corporation
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- KOIKE-TAKESHITA Ayumi
- Bio Research Laboratory, Toyota Motor Corporation
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- YOKOYAMA Ken
- Bio Research Laboratory, Toyota Motor Corporation
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- MURAMATSU Masayoshi
- Bio Research Laboratory, Toyota Motor Corporation
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- NISHINO Tokuzo
- Department of Biochemistry and Engineering, Tohoku University
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- OBATA Shusei
- Bio Research Laboratory, Toyota Motor Corporation
書誌事項
- タイトル別名
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- Gene Cloning and Overexpression of a Geranylgeranyl Diphosphate Synthase of an Extremely Thermophilic Bacterium, Thermus thermophilus.
- Gene Cloning and Overexpression of a Geranylgeranyl Diphosphate Synthase of an Extremely Thermophilic Bacterium,<i>Thermus thermophilus</i>
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説明
A geranylgeranyl diphosphate (GGPP) synthase gene of an extremely thermophilic bacterium, Thermus thermophilus, was cloned and sequenced. T. thermophilus GGPP synthase, overexpressed in Escherichia coli cells as a glutathione S-transferase fusion protein, was purified and characterized. The fusion protein, retaining thermostability, formed a homodimer, and showed higher specific activity than did a partially purified thermostable enzyme previously reported. Optimal reaction conditions and kinetic parameters were also examined. The deduced amino acid sequence indicated that T. thermophilus GGPP synthase was excluded from the group of bacterial type GGPP synthases and lacked the insertion amino acid residues in the first aspartate-rich motif as do archaeal and eukaryotic short-chain prenyltransferases.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 63 (2), 261-270, 1999
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681452431104
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- NII論文ID
- 110002679460
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1MXhs12gt7o%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4676557
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- PubMed
- 10192906
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可