Addition of a Peptide Tag at the C Terminus of AtHKT1 Inhibits its Na〔+〕 Transport

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  • KATO Yasuhiro
    <i>Graduate School of Bioagricultural Sciences, Nagoya University</i>
  • HAZAMA Akihiro
    <i>Fukushima Medical University</i>
  • YAMAGAMI Mutsumi
    <i>Institute of Environmental Sciences</i>
  • UOZUMI Nobuyuki
    <i>Graduate School of Bioagricultural Sciences, Nagoya University</i> <i>Bioscience and Biotechnology Center, Nagoya University</i>

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  • Addition of a Peptide Tag at the C Terminus of AtHKT1 Inhibits Its Na+ Transport
  • Addition of a Peptide Tag at the C Terminus of AtHKT1 Inhibits Its Na<sup>+</sup>Transport

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  Eight transmembrane segments in the Arabidopsis Na+ transporter, AtHKT1, have been proposed to be arranged around the axis of the pore. We report here that the addition of a GFP or a FLAG tag to the C terminus of AtHKT1 impairs the Na+ transport in Xenopus ooytes as detected by electrophysiological measurements.<br>

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