Purification and Characterization of an Endo-Polygalacturonase from a Mutant of<i>Saccharomyces cerevisiae</i>
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- HIROSE Naoto
- Department of Applied Biochemistry, Faculty of Agriculture, Osaka Prefecture University
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- KISHIDA Masao
- Department of Applied Biochemistry, Faculty of Agriculture, Osaka Prefecture University
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- KAWASAKI Haruhiko
- Department of Applied Biochemistry, Faculty of Agriculture, Osaka Prefecture University
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- SAKAI Takuo
- Department of Food Science, Faculty of Agriculture, Kinki University
書誌事項
- タイトル別名
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- Purification and Characterization of an Endo-Polygalacturonase from a Mutant of Saccharomyces cerevisiae.
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説明
An extracellular endo-polygalacturonase (PGase) produced by a mutant of Saccharomyces cerevisiae was isolated. The enzyme was regarded, immunologically, as a PGase belonging to the Kluyveromyces marxianus group. The enzyme had properties similar to the PGase from K. marxianus in heat and pH stability, and N-terminal amino acid sequence. However, the enzyme showed different properties in optimum pH and temperature, molecular weight, and reactivity in antiserum against PGase from K. marxianus, indicating that the enzyme has a different molecular structure from the PGase from K. marxianus.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 63 (6), 1100-1103, 1999
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681452700416
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- NII論文ID
- 110002679624
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1MXkt12gurs%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4787174
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- PubMed
- 10427698
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可