Visualization of Biallelic Expression of the Imprinted SNRPN Gene Induced by Inhibitors of DNA Methylation and Histone Deacetylation.

DOI Web Site Web Site PubMed 32 References
  • KOHDA Atsushi
    Laboratory of Molecular and Cellular Biology, Faculty of Bioresources, Mie University
  • TAGUCHI Hiroshi
    Laboratory of Molecular and Cellular Biology, Faculty of Bioresources, Mie University
  • OKUMURA Katsuzumi
    Laboratory of Molecular and Cellular Biology, Faculty of Bioresources, Mie University

Search this article

Description

We have adapted a fluorescence in situ hybridization (FISH) method to detect nascent RNA molecules of the imprinted SNRPN gene at the initial transcription sites in nuclei of human HL60 and WI38 cells. Simultaneous detection of RNA and DNA of SNRPN by FISH using the cosmid probe confirmed its monoallelic expression in these cell lines. Treatment of both cells by inhibitors of DNA methylation and histone deacetylation resulted in time-dependent increase of the cell population with the biallelic expression of SNRPN.

Journal

References(32)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top