Expression of Biologically Active Human Calpastatin in Baculovirus-infected Insect Cells and in<i>Escherichia coli</i>
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- HITOMI Kiyotaka
- School of Agricultural Sciences, Nagoya University
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- YOKOYAMA Akiko
- School of Agricultural Sciences, Nagoya University
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- MAKI Masatoshi
- School of Agricultural Sciences, Nagoya University
書誌事項
- タイトル別名
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- Expression of Biologically Active Human Calpastatin in Baculovirus-infected Insect Cells and in Escherichia coli.
- Expression of Biologically Active Human
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説明
Calpastatin, an endogeneous inhibitor protein acting on calpain (Ca2+-dependent cysteine proteinase), is widely distributed in animal tissues and cells. Two different expression systems, baculovirus-infected Spodoptera frugiperda (Sf9) insect cells and Escherichia coli, were used for overexpression of the human calpastatin tagged with N-terminal hexahistidine peptide. Recombinant calpastatin was purified to homogeneity by nickel ion affinity chromatography and gel filtration separation. Purified recombinant proteins from both systems have similar inhibitory activity for calpain.<br>
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 62 (1), 136-141, 1998
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681453059328
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- NII論文ID
- 110002678929
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1cXpsVCqsw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4406236
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- PubMed
- 9501525
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可
