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Expression of Biologically Active Human Calpastatin in Baculovirus-infected Insect Cells and in Escherichia coli.
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- HITOMI Kiyotaka
- School of Agricultural Sciences, Nagoya University
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- YOKOYAMA Akiko
- School of Agricultural Sciences, Nagoya University
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- MAKI Masatoshi
- School of Agricultural Sciences, Nagoya University
Bibliographic Information
- Other Title
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- Expression of Biologically Active Human
- Expression of Biologically Active Human Calpastatin in Baculovirus-infected Insect Cells and in<i>Escherichia coli</i>
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Description
Calpastatin, an endogeneous inhibitor protein acting on calpain (Ca2+-dependent cysteine proteinase), is widely distributed in animal tissues and cells. Two different expression systems, baculovirus-infected Spodoptera frugiperda (Sf9) insect cells and Escherichia coli, were used for overexpression of the human calpastatin tagged with N-terminal hexahistidine peptide. Recombinant calpastatin was purified to homogeneity by nickel ion affinity chromatography and gel filtration separation. Purified recombinant proteins from both systems have similar inhibitory activity for calpain.<br>
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 62 (1), 136-141, 1998
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681453059328
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- NII Article ID
- 110002678929
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1cXpsVCqsw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 4406236
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- PubMed
- 9501525
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- JaLC
- NDL Search
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed