Expression of the Cellulase (FI-CMCase) Gene of<i>Aspergillus aculeatus</i>in<i>Saccharomyces cerevisiae</i>
書誌事項
- タイトル別名
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- Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Saccharomyces cerevisiae.
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説明
As a step to breed a Saccharomyces cerevisiae strain able to produce ethanol directly from cellulose, we combined cDNA for Aspergillus aculeatus FI-CMCase (FI-carboxymethyl cellulase) with the GAP (glyceraldehyde-3-phosphate dehydrogenase) promoter of S. cerevisiae and used the resultant plasmid, pYEC91, to transform S. cerevisiae. The transformed cells produced active FI-CMCase within the cytoplasm. Western-blot analysis following SDS-polyacrylamide gel electrophoresis demonstrated that the cells contained a peptide having the same molecular mass and immunological identity as A. aculeatus FI-CMCase.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 58 (5), 954-956, 1994
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681453088256
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- NII論文ID
- 110002676984
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK2cXktlSru7c%3D
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- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
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- PubMed
- 7764982
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
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- PubMed
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