Purification, Characterization, and Antifungal Activity of Chitinases from Pineapple (Ananas comosus) Leaf

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  • TAIRA Toki
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University
  • TOMA Noriko
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University
  • ISHIHARA Masanobu
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University

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  • Purification, Characterization, and Antifungal Activity of Chitinases from Pineapple (<i>Ananas comosus</i>) Leaf

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Abstract

Three chitinases, designated pineapple leaf chitinase (PL Chi)-A, -B, and -C were purified from the leaves of pineapple (Ananas comosus) using chitin affinity column chromatography followed by several column chromatographies. PL Chi-A is a class III chitinase having a molecular mass of 25 kDa and an isoelectric point of 4.4. PL Chi-B and -C are class I chitinases having molecular masses of 33 kDa and 39 kDa and isoelectric points of 7.9 and 4.6 respectively. PL Chi-C is a glycoprotein and the others are simple proteins. The optimum pHs of PL Chi-A, -B, and -C toward glycolchitin are pH 3, 4, and 9 respectively. The chitin-binding ability of PL Chi-C is higher than that of PL Chi-B, and PL Chi-A has lower chitin-binding ability than the others. At low ionic strength, PL Chi-B exhibits strong antifungal activity toward Trichoderma viride but the others do not. At high ionic strength, PL Chi-B and -C exhibit strong and weak antifungal activity respectively. PL Chi-A does not have antifungal activity.

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