Cloning, Sequencing, and Expression of a<i>β</i>-Amylase Gene from<i>Bacillus cereus</i>var.<i>mycoides</i>and Characterization of Its Products
書誌事項
- タイトル別名
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- Cloning, Sequencing, and Expression of a .BETA.-Amylase Gene from Bacillus cereus var. mycoides and Characterization of Its Products.
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説明
The cloned gene was composed of 1638 bp for coding plus promoter like and SD-like sequences ahead of it. The deduced amino acid sequence had high similarity with known β-amylases. The N-terminal sequence of the cloned β-amylase seemed to be a signal peptide. The gene was introduced into Bacillus subtilis 1A289 using pHY300PLK as a vector and the expressed protein was recovered from the culture media. The enzyme fraction produced was divided into two components upon the DEAE column chromatography. The amino acid sequence of one fraction (FrI) was the same as the mature enzyme, and the other (FrII) lacked the N-terminal amino acid residue (Ala) of the mature enzyme. The kinetic parameters of the hydrolysis catalyzed by the enzyme component fir were measured, and the subsite affinities of the enzyme were evaluated. In conclusion, it was shown that the recombinant enzyme was the same as the mature enzyme functionally and proteochemically.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 60 (8), 1255-1259, 1996
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681453303808
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- NII論文ID
- 110002678240
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK28XltlektLo%3D
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- ISSN
- 13476947
- 09168451
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- PubMed
- 8987540
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可