No Factors Except for the Hap Complex increase the Taka-amylase A Gene Expression by Binding to the CCAAT Sequence in the Promoter Region.

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  • KATO Masashi
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • NARUSE Fumiko
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • KOBAYASHI Tetsuo
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • TSUKAGOSHI Norihiro
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

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To examine the possibility that factors different from the Hap complex are involved in increasing Taka-amylase A gene (taa) expression in Aspergillus nidulans, either the authentic taa gene or the mutant taa gene with a replacement of the CCAAT box was expressed in either a wild type strain or a mutant strain lacking the functional Hap complex (hapCΔ). When the mutant taa was expressed in the hapCΔ strain, enzyme activity was as low as that of the hapCΔ strain transformed with the authentic taa gene, indicating that no factors except for the Hap complex increase transcription of the taa gene by binding to the CCAAT sequence.

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