Persimmon Leaf Extract Inhibits the ATM Activity during DNA Damage Response Induced by Doxorubicin in A549 Lung Adenocarcinoma Cells
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- KAWAKAMI Kayoko
- Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences
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- NISHIDA Hiroshi
- Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences
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- TATEWAKI Naoto
- Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences
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- NAKAJIMA Yuki
- Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences
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- KONISHI Tetsuya
- Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences
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- HIRAYAMA Masao
- Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences
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Persimmon leaf (PL) has been commonly recognized for its wide variety of health benefits. A previous study has reported that persimmon leaf extract (PLE) contained flavonols with the 2″-galloly moiety (PLEg). Galloylated homologues generically show stronger activity in their biological function, so enhanced functions can be expected for PLEg. We investigated in this present study the effect of PLEg on the cellular DNA damage checkpoint signaling to sensitize cancer chemotherapy. Treatment with PLE and PLEg significantly increased the cytotoxicity of doxorubicin (DOX) in A549 adenocarcinoma cells. PLE and PLEg reduced the phosphorylation of checkpoint proteins such as structural maintenance of chromosomes 1 (SMC1), checkpoint kinase 1 (Chk1), and p53 in DOX-treated cells. Moreover, PLE decreased the phosphorylation of ATM (ataxia telangiectasia mutated) in a dose-dependent manner. PLE, and especially PLEg, abrogated the G2/M checkpoint during DOX-induced DNA damage. These results suggest that PLEg specifically inhibited ATM-dependent checkpoint activation by DOX, and that PLEg might be a useful sensitizer in cancer chemotherapy.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (4), 650-655, 2011
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681453623040
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- NII論文ID
- 10028271254
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 11070916
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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