Oviduct-Specific Enhanced Green Fluorescent Protein Expression in Transgenic Chickens

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  • BYUN Sung June
    Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration
  • KIM Sung Woo
    Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration
  • KIM Kyung-Woon
    Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration
  • KIM Jeom Sun
    Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration
  • HWANG In-Sul
    Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration Interdisciplinary Graduate School of Science and Technology, Shinshu University
  • CHUNG Hee Kyoung
    Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration
  • KAN In Sun
    Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration
  • JEON Ik-Soo
    Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration
  • CHANG Won-Kyoung
    Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration
  • PARK Soo-Bong
    Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration
  • YOO Jae Gyu
    Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration

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In this study, we confirmed the ability of the 2-kb promoter fragment of the chicken ovalbumin gene to drive tissue-specific expression of a foreign EGFP gene in chickens. Recombinant lentiviruses containing the EGFP gene were injected into the subgerminal cavity of 539 freshly laid embryos (stage X). Subsequently the embryos were incubated to hatch using phases II and III of the surrogate shell ex vivo culture system. Twenty-four chicks (G0) were hatched and screened for EGFP with PCR. Two chicks were identified as transgenic birds (G1), and these founders were mated with wild-type chickens to generate transgenic progeny. In the generated transgenic hens (G2), EGFP was expressed specifically in the tubular gland of the oviduct. These results show the potential of the chicken ovalbumin promoter for the production of biologically active proteins in egg white.

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