Immunostimulation Effects of Yellowtail Heart Extracts<i>in Vitro</i>and<i>in Vivo</i>
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- DAIFUKU Miho
- Faculty of Agriculture, Ehime University
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- YAGUCHI Yusuke
- Faculty of Agriculture, Ehime University
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- NISHI Kosuke
- Faculty of Agriculture, Ehime University
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- OKAMOTO Takeaki
- Kawasaki Medical University
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- NAKANO Hiroyuki
- Kawasaki Medical University
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- NISHIMOTO Sogo
- Center for Marine Environmental Studies, Ehime University
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- NISHIKAWA Satoshi
- Fisheries Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries
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- YAMASHITA Hirofumi
- Fisheries Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries
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- TAKIMOTO Shinichi
- Fisheries Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries
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- SUGAHARA Takuya
- Faculty of Agriculture, Ehime University South Ehime Fisheries Research Center, Ehime University
書誌事項
- タイトル別名
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- Immunostimulation Effects of Yellowtail Heart Extracts in Vitro and in Vivo
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抄録
The immunostimulation effects of yellowtail heart extracts were examined. Screening various parts of the yellowtail viscera, we found that extracts from the yellowtail heart enhanced IgM production by human hybridoma HB4C5 cells. Yellowtail heart extracts heated at 121 °C for 20 min and dialyzed showed the highest IgM production-stimulating activity toward HB4C5 cells. Also, immunoglobulin production by mouse spleen lymphocytes was stimulated by yellowtail heart extracts in vitro, and lymphocytes derived from mice administered the extract for 20 d were activated in vivo. Yellowtail heart extracts were partially purified by anion-exchange chromatography, and fractions containing a 33 kDa-protein exhibited immunostimulating activity. LC-MS/MS analysis revealed that the 33 kDa-protein was most similar to tropomyosin-4 from various fishes. Purified tropomyosin from porcine muscle enhanced IgM production by HB4C5 cells. This means that tropomyosin-4 is one of the immunostimulating substances in the yellowtail heart.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (4), 638-645, 2011
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681453639936
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- NII論文ID
- 10028271208
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 11070902
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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