Oligomerization and DNA-Binding Capacity of Pmr, a Histone-Like Protein H1 (H-NS) Family Protein Encoded on IncP-7 Carbazole-Degradative Plasmid pCAR1
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- SUZUKI Chiho
- Biotechnology Research Center, The University of Tokyo
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- YUN Choong-Soo
- Biotechnology Research Center, The University of Tokyo Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- UMEDA Takashi
- Biotechnology Research Center, The University of Tokyo
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- TERABAYASHI Tsuguno
- Biotechnology Research Center, The University of Tokyo
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- WATANABE Kazuya
- Research Center for Advanced Science and Technology, The University of Tokyo
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- YAMANE Hisakazu
- Biotechnology Research Center, The University of Tokyo
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- NOJIRI Hideaki
- Biotechnology Research Center, The University of Tokyo Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Pmr, a histone-like protein H1 (H-NS) family protein encoded on plasmid pCAR1, is a key factor in optimizing gene transcription on both pCAR1 and the host chromosome. To clarify the mode of function of Pmr, we performed gel filtration chromatography analysis and protein-protein cross-linking, and found that Pmr forms homo-oligomers, consisting of its homodimers. We also found, by atomic force microscopy, that Pmr has DNA-bridging capacity. From these results, Pmr was deduced to have features common to H-NS family proteins. Additionally, evaluating protein-DNA affinity is important to clarify the mode of function of Pmr, and hence we performed an electrophoretic mobility shift assay. Though Pmr formed high-order protein-DNA complexes and did not show preference for nucleic acid sequences, the C-terminal region of Pmr did, suggesting that the DNA-binding affinity of Pmr can be evaluated by using its C-terminal region.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (4), 711-717, 2011
公益社団法人 日本農芸化学会
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- CRID
- 1390282681453643776
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- NII論文ID
- 10028271550
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 11071047
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- 本文言語コード
- en
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