The<i>in Vivo</i>and<i>in Vitro</i>Stimulatory Effects of Cordycepin on Mouse Leydig Cell Steroidogenesis
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- LEU Sew-Fen
- Department of Food Science and Technology, College of Human Ecology, Hung Kuang University
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- POON Song Ling
- Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University
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- PAO Hsiang-Yin
- Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University
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- HUANG Bu-Miin
- Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University
書誌事項
- タイトル別名
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- The in Vivo and in Vitro Stimulatory Effects of Cordycepin on Mouse Leydig Cell Steroidogenesis
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抄録
Cordycepin, a pure compound of Cordyceps sinensis (CS), is known as an adenosine analog. We have found that CS stimulated Leydig cell steroidogenesis. Here we investigated the in vivo and in vitro effects of cordycepin in primary mouse Leydig cell steroidogenesis. The results indicate that cordycepin increased the plasma testosterone concentration. Cordycepin also stimulated in vitro mouse Leydig cell testosterone production in dose- and time-dependent manners. We further observed that cordycepin regulated the mRNA expression of the A1, A2a, A2b, and A3 adenosine receptors in the mouse Leydig cells, and that antagonists of A1, A2a, and A3 suppressed testosterone production 20–50% testosterone production. Furthermore, Rp-cAMPS (cAMP antagonist) and Protein Kinase A (PKA) inhibitors (H89 and PKI) significantly decreased cordycepin-induced testosterone production, indicating that the PKA-cAMP signal pathway was activated by cordycepin through adenosine receptors. Moreover, cordycepin induced StAR protein expression, and H89 suppressed cordycepin-induced steroidogenic acute regulatory (StAR) protein expression. Conclusively, cordycepin associated with adenosine receptors to activate cAMP-PKA-StAR pathway and steroidogenesis in the mouse Leydig cells.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (4), 723-731, 2011
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681453646080
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- NII論文ID
- 10028271606
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 11071058
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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