High yield synthesis of 12-aminolauric acid by "enzymatic transcrystallization" of ω-laurolactam using ω-laurolactam hydrolase from Acidovorax sp. T31
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- FUKUTA Yasuhisa
- Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University
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- KOMEDA Hidenobu
- Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University
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- YOSHIDA Yoichi
- Research and Development Headquarters of Ubekousan, Ubekousan
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- ASANO Yasuhisa
- Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University
書誌事項
- タイトル別名
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- High Yield Synthesis of 12-Aminolauric Acid by "Enzymatic Transcrystallization" of .OMEGA.-Laurolactam Using .OMEGA.-Laurolactam Hydrolase from Acidovorax sp. T31
- High yield synthesis of 12 aminolauric acid by enzymatic transcrystallization of o laurolactam using o laurolactam hydrolase from Acidovorax sp T31
- High Yield Synthesis of 12-Aminolauric Acid by “Enzymatic Transcrystallization” of ω-Laurolactam Using ω-Laurolactam Hydrolase from<i>Acidovorax</i>sp. T31
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抄録
The genes encoding ω-laurolactam hydrolases from Cupriavidus sp. T7, Acidovorax sp. T31, Cupriavidus sp. U124, and Sphingomonas sp. U238 were cloned and sequenced. Nucleotide and amino acid sequence analysis of the four genes indicated that the primary structures of these ω-laurolactam hydrolases are significantly similar to the 6-aminohexanoate-cyclic-dimer hydrolase (EC 3.5.2.12). These genes were expressed in Escherichia coli, and the ω-laurolactam hydrolysing activity of the recombinant enzymes was compared with that of 6-aminohexanoate-cyclic-dimer hydrolase from Arthrobacter sp. KI72. The enzyme from Acidovorax sp. T31 was most successfully expressed in E. coli. Cell-free extract of the recombinant strain was used for the synthesis of 12-aminolauric acid from ω-laurolactam by “enzymatic transcrystallization,” because crystalline ω-laurolactam added into the enzyme solution was converted to crystalline 12-aminolauric acid (≧97.3% yield). Under the optimum conditions, 208 g/l of 12-aminolauric acid was produced in 17 h. The resulting pure product was identical to authentic 12-aminolauric acid.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 73 (5), 980-986, 2009
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681453687040
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- NII論文ID
- 10027540396
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 10234407
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可
