High Shikimate Production from Quinate with Two Enzymatic Systems of Acetic Acid Bacteria
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- ADACHI Osao
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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- ANO Yoshitaka
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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- TOYAMA Hirohide
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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- MATSUSHITA Kazunobu
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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Abstract
3-Dehydroshikimate was formed with a yield of 57–77% from quinate via 3-dehydroquinate by two successive enzyme reactions, quinoprotein quinate dehydrogenase (QDH) and 3-dehydroquinate dehydratase, in the cytoplasmic membranes of acetic acid bacteria. 3-Dehydroshikimate was then reduced to shikimate (SKA) with NADP-dependent SKA dehydrogenase (SKDH) from the same organism. When SKDH was coupled with NADP-dependent D-glucose dehydrogenase (GDH) in the presence of excess D-glucose as an NADPH re-generating system, SKDH continued to produce SKA until 3-dehydroshikimate added initially in the reaction mixture was completely converted to SKA. Based on the data presented, a strategy for high SKA production was proposed.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (10), 2579-2582, 2006
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681453735168
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- NII Article ID
- 10018526303
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 8524736
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed