Identification of a Functional 2-keto-<i>myo</i>-Inositol Dehydratase Gene of<i>Sinorhizobium fredii</i>USDA191 Required for<i>myo</i>-Inositol Utilization
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- YOSHIDA Ken-ichi
- Department of Biofunctional Chemistry, Kobe University
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- KIM Won-Seok
- Department of Agronomy, University of Missouri
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- KINEHARA Masaki
- Department of Biofunctional Chemistry, Kobe University
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- MUKAI Rie
- Department of Biofunctional Chemistry, Kobe University
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- ASHIDA Hitoshi
- Department of Biofunctional Chemistry, Kobe University
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- IKEDA Hideki
- Department of Biotechnology, Fukuyama University
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- FUJITA Yasutaro
- Department of Biotechnology, Fukuyama University
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- KRISHNAN Hari B.
- Department of Agronomy, University of Missouri Plant Genetic Research Unit, USDA-ARS, University of Missouri
書誌事項
- タイトル別名
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- Identification of a Functional 2-keto-myo-Inositol Dehydratase Gene of Sinorhizobium fredii USDA191 Required for myo-Inositol Utilization
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説明
Sinorhizobium fredii USDA191 is a Gram-negative bacterium capable of forming nitrogen-fixing nodules on soybean roots. The USDA191 idhA gene encoding myo-inositol dehydrogenase, an enzyme necessary for myo-inositol utilization, is known to be involved in competitive nodulation and nitrogen fixation. In Bacillus subtilis, myo-inositol dehydrogenase catalyzes the first step of the myo-inositol catabolic pathway. Recently iolE was identified as the gene encoding 2-keto-myo-inositol dehydratase, which catalyzes the second step in the pathway. Here we report the presence of 2-keto-myo-inositol dehydratase activity in free-living USDA191 cells cultured in a medium containing myo-inositol. An iolE ortholog was cloned from USDA191. USDA191 iolE was expressed in Escherichia coli as a His6-tag fusion and purified to exhibit 2-keto-myo-inositol dehydratase activity. Inactivation of USDA191 iolE led to defective myo-inositol utilization. USDA191 iolE partially complemented a B. subtilis iolE deficient mutant. These results suggest that S. fredii USDA191 utilizes a myo-inositol catabolic pathway, analogous to that of B. subtilis, involving at least idhA and iolE.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 70 (12), 2957-2964, 2006
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681453785984
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- NII論文ID
- 10018523881
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 8596534
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- PubMed
- 17151471
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- 本文言語コード
- en
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- 資料種別
- journal article
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