Immunological Characterization of Honey Proteins and Identification of MRJP 1 as an IgE-Binding Protein

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  • HAYASHI Takeshi
    Department of Food and Fermentation Science, Faculty of Food and Nutrition, Beppu University
  • TAKAMATSU Nobue
    Department of Food and Fermentation Science, Faculty of Food and Nutrition, Beppu University
  • NAKASHIMA Takashi
    Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
  • ARITA Takashi
    Ehime Seikyou Hospital

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We encountered a fourth case of honey allergy in Japan. We characterized and identified the IgE-binding proteins in honey using the serum of a honey-allergenic patient. Immunoblot analysis revealed that IgE in the patient serum specifically bound to four proteins in each honey sample. At least three of these IgE-binding proteins were N-linked glycoproteins. To identify the 60-kDa IgE-binding protein in dandelion honey, the N-terminal sequences of the fragmented protein were analyzed, revealing the protein to be major royal jelly protein 1 (MRJP 1). Three IgE-binding proteins removed of N-linked oligosaccharide showed a large reduction in IgE-binding activity as compared with the intact protein. This suggests that the carbohydrates in the IgE-binding proteins are a major epitope for patient IgE.

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