Streptavidin-Aequorin Fusion Protein for Bioluminescent Immunoassay
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- INOUYE Satoshi
- Yokohama Research Center, Chisso Corporation
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- SATO Jun-ichi
- Yokohama Research Center, Chisso Corporation
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- SASAKI Satoko
- Yokohama Research Center, Chisso Corporation
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- SAHARA Yuiko
- Yokohama Research Center, Chisso Corporation
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The fusion protein of streptavidin to aequorin (STA-AQ) was highly purified from inclusion bodies in Escherichia coli cells and applied to a bioluminescent sandwich immunoassay. α-Fetoprotein (AFP), which is a serological marker of liver cancer, was used as a model analyte to test STA-AQ in an immunoassay. The measurable range of AFP by the sandwich immunoassay, using the complex of STA-AQ and the biotinylated anti-AFP antibody, was 0.02–200 ng/mL with an average coefficient of variation of 4.9%. The detection sensitivity with the complex of STA-AQ and the biotinylated anti-AFP antibody was similar to that with the complex of biotinylated aequorin, streptavidin and the biotinylated anti-AFP antibody. STA-AQ would be a useful reporter protein for immunoassays.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (3), 568-571, 2011
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681453808896
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- NII論文ID
- 10028202035
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 11051853
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可