Highly Sensitive Quantitative Analysis of Nicotianamine Using LC/ESI-TOF-MS with an Internal Standard

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  • WADA Yasuaki
    Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • YAMAGUCHI Isomaro
    Faculty of Technology, Maebashi Institute of Technology
  • TAKAHASHI Michiko
    Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • NAKANISHI Hiromi
    Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • MORI Satoshi
    Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • NISHIZAWA Naoko K.
    Graduate School of Agricultural and Life Sciences, The University of Tokyo Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST)

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説明

A highly sensitive quantitative method for analyzing nicotianamine (NA) by liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) is reported. Fluorenylmethoxycarbonylation of nicotianamine reduced its polarity and enabled its retention in a reversed-phase column. The adoption of Nε-nicotyllysine (NL) as an internal standard ensured reliable quantification by giving a linear calibration curve drawn between the NA/NL molar ratios of standard solutions injected and the NA/NL area ratios in mass chromatograms. The high sensitivity of this analytical method allowed us to measure the amount of NA. This analytical method has applications to all research concerning NA.

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