Development of a Novel Functional High-Throughput Screening System for Pathogen Effectors in the Yeast<i>Saccharomyces cerevisiae</i>
-
- TABUCHI Mitsuaki
- Department of Molecular Genetics, Kawasaki Medical School
-
- KAWAI Yasuhiro
- Department of Pediatrics, Kawasaki Medical School
-
- NISHIE-FUJITA Makiko
- Department of Molecular Genetics, Kawasaki Medical School
-
- AKADA Rinji
- Department of Applied Molecular Bioscience, Division of Engineering, Yamaguchi University
-
- IZUMI Terumi
- Department of Molecular Genetics, Kawasaki Medical School
-
- YANATORI Izumi
- Department of Molecular Genetics, Kawasaki Medical School
-
- MIYASHITA Naoyuki
- Department of Respiratory Diseases, Kawasaki Medical School
-
- OUCHI Kazunobu
- Department of Pediatrics, Kawasaki Medical School
-
- KISHI Fumio
- Department of Molecular Genetics, Kawasaki Medical School
書誌事項
- タイトル別名
-
- Development of a Novel Functional High-Throughput Screening System for Pathogen Effectors in the Yeast Saccharomyces cerevisiae
この論文をさがす
抄録
Our understanding of the molecular mechanisms of bacterial pathogenesis has been improved especially by the discovery of host cell contact-dependent secretion systems such as the type-III secretion system (T3SS) found in numerous pathogens. Although the identification of pathogen effectors translocated into host cells through T3SS is essential to the understanding of pathogenesis, their general sequence uniqueness confound attempts to identify such proteins by sequence homology. Here we report the development of a functional high-throughput screening system for pathogen effectors in yeast that consists of a GatewayTM-compatible Tet-Off inducible expression vector and a yeast strain expressing a reporter, facilitating identification of the effectors affecting host vesicular trafficking pathways. We evaluated this system and optimized the screening condition using several known pathogen effectors. We found this system useful in functional characterization of pathogen effector and it can be adapted to functional high-throughput screening as well.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 73 (10), 2261-2267, 2009
公益社団法人 日本農芸化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681454099456
-
- NII論文ID
- 10027546838
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/13476947
-
- NDL書誌ID
- 10419268
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可