Characterization of Recombinant .BETA.-Amylases from Oryza sativa
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- KOIDE Tomojiro
- Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo
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- OHNISHI Yasuo
- Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo
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- HORINOUCHI Sueharu
- Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo
Bibliographic Information
- Other Title
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- Characterization of recombinant β-amylases from Oryza sativa
- Characterization of recombinant v amylases from Oryza sativa
- Characterization of Recombinant β-Amylases from<i>Oryza sativa</i>
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Abstract
Four putative β-amylase genes found in the Oryza sativa cDNA sequence database (KOME) were expressed in Escherichia coli. Recombinant proteins from two of these genes showed β-amylase activity. Similarly to β-amylases from other plants, the optimum pH of the recombinant rice β-amylases was about 5.5–6.0, but they exhibited inferior heat stability to soybean β-amylase.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (4), 793-796, 2011
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681454350720
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- NII Article ID
- 10028271987
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 11071210
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed