Isolation and Characterization of<i>Acidithiobacillus ferrooxidans</i>Strain D3-2 Active in Copper Bioleaching from a Copper Mine in Chile
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- SUGIO Tsuyoshi
- Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
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- WAKABAYASHI Masanori
- Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
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- KANAO Tadayoshi
- Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
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- TAKEUCHI Fumiaki
- Environmental Management and Safety Section, Health and Environment Center, Okayama University
書誌事項
- タイトル別名
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- Isolation and Characterization of Acidithiobacillus ferrooxidans Strain D3-2 Active in Copper Bioleaching from a Copper Mine in Chile
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Acidithiobacillus ferrooxidans strain D3-2, which has a high copper bioleaching activity, was isolated from a low-grade sulfide ore dump in Chile. The amounts of Cu2+ solubilized from 1% chalcopyrite (CuFeS2) concentrate medium (pH 2.5) by A. ferrooxidans strains D3-2, D3-6, and ATCC 23270 and 33020 were 1360, 1080, 650, and 600 mg·l−1·30 d−1. The iron oxidase activities of D3-2, D3-6, and ATCC 23270 were 11.7, 13.2, and 27.9 μl O2 uptake·mg protein−1·min−1. In contrast, the sulfite oxidase activities of strains D3-2, D3-6, and ATCC 23270 were 5.8, 2.9, and 1.0 μl O2 uptake·mg protein−1·min−1. Both of cell growth and Cu-bioleaching activity of strains D3-6 and ATCC 23270, but not, of D3-2, in the chalcopyrite concentrate medium were completely inhibited in the presence of 5 mM sodium bisulfite. The sulfite oxidase of strain D3-2 was much more resistant to sulfite ion than that of strain ATCC 23270. Since sulfite ion is a highly toxic intermediate produced during sulfur oxidation that strongly inhibits iron oxidase activity, these results confirm that strain D3-2, with a unique sulfite resistant-sulfite oxidase, was able to solubilize more copper from chalcopyrite than strain ATCC 23270, with a sulfite-sensitive sulfite oxidase.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 72 (4), 998-1004, 2008
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681454417536
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- NII論文ID
- 10027526575
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 9481158
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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