Development of a Series of Gateway Binary Vectors Possessing a Tunicamycin Resistance Gene as a Marker for the Transformation of<i>Arabidopsis thaliana</i>

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  • TANAKA Yuji
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
  • NAKAMURA Shinya
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
  • KAWAMUKAI Makoto
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
  • KOIZUMI Nozomu
    Graduate School of Life and Environmental Sciences, Osaka Prefecture University
  • NAKAGAWA Tsuyoshi
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University

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  • Development of a Series of Gateway Binary Vectors Possessing a Tunicamycin Resistance Gene as a Marker for the Transformation of Arabidopsis thaliana

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We made two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing a UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene driven by the nopaline synthase promoter (Pnos) as a tunicamycin resistance marker for the transformation of Arabidopsis thaliana. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7, and TAP. Selection of transformants was successful on plates containing 0.15 mg/L of tunicamycin. These vectors were compatible with existing pGWB and R4pGWB vectors for kanamycin, hygromycin B, and BASTA® selection, and are useful new tools for making transgenic Arabidopsis.

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