An Adhesin-Like Protein, Lam29, from Lactobacillus mucosae ME-340 Binds to Histone H3 and Blood Group Antigens in Human Colonic Mucus
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- WATANABE Masamichi
- Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
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- KINOSHITA Hideki
- School of Food, Agricultural and Environmental Sciences, Miyagi University School of Food, Agricultural and Environmental Sciences, Miyagi University
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- HUANG I-Nung
- Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
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- EGUCHI Kei
- Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
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- TSURUMI Takuya
- Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
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- KAWAI Yasushi
- Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
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- KITAZAWA Haruki
- Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
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- KIMURA Katsunori
- Division of Research and Development, Meiji Dairies Corporation Division of Research and Development, Meiji Dairies Corporation
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- TAKETOMO Naoki
- Division of Research and Development, Meiji Dairies Corporation Division of Research and Development, Meiji Dairies Corporation
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- KIKUCHI Daisuke
- Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine
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- SASE Tomohiko
- Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine
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- MIURA Koh
- Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine
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- OGAWA Hitoshi
- Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine
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- SHIBATA Chikashi
- Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine
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- HORII Akira
- Department of Molecular Pathology, Tohoku University School of Medicine Department of Molecular Pathology, Tohoku University School of Medicine
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- SAITO Tadao
- Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
書誌事項
- タイトル別名
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- An Adhesin-Like Protein, Lam29, from <i>Lactobacillus mucosae</i> ME-340 Binds to Histone H3 and Blood Group Antigens in Human Colonic Mucus
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抄録
A cell-surface 29-kDa protein (Lam29, cysteine-binding protein of the ABC transporter) from Lactobacillus mucosae ME-340 showed an adhesin-like property for human ABO blood group antigens expressed on the gastrointestinal mucosa. In addition, here we report that Lam29 also bound to an 18-kDa protein on human colonic mucus. By ligand blot assay and N-terminal amino acid sequence of the protein, it was identified as human histone H3. By ligand blot and microplate binding assays with recombinant histone H3, binding between Lam29 and histone H3 was confirmed. The adhesion of ME-340 cells to histone H3 was significantly inhibited by 26% after the addition of 2.5 mg/mL Lam29 as compared to the absence of Lam29 (p<0.01). By GHCl extraction and transcription attenuation of ME-340 cells, binding reduction of ME340 cells against histone H3 was detected at 12% and 13% respectively, as compared to control cells by the BIACORE assay (p<0.01). These data indicate that Lam29 shows multiple binding activities to blood group antigens and histone H3 in human colonic mucus. This is the first report to indicate that lactobacilli expressing Lam29 adhere to histone H3 on gastrointestinal mucosa.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 76 (9), 1655-1660, 2012
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681454652288
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- NII論文ID
- 130004137955
- 10031123168
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC38bmsVWiug%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 023966122
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- PubMed
- 22972326
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可