Dual-Fluorescent RNA Probes with an Extremely Large Stokes Shift

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  • KOBORI Akio
    Kyoto Institute of Technology, Graduate School of Science and Technology, Department of Biomolecular Engineering
  • UEDA Takako
    Kyoto Institute of Technology, Graduate School of Science and Technology, Department of Biomolecular Engineering
  • SANADA Yuya
    Kyoto Institute of Technology, Graduate School of Science and Technology, Department of Biomolecular Engineering
  • YAMAYOSHI Asako
    Kyoto Institute of Technology, Graduate School of Science and Technology, Department of Biomolecular Engineering
  • MURAKAMI Akira
    Kyoto Institute of Technology, Graduate School of Science and Technology, Department of Biomolecular Engineering

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Abstract

Fluorescent probes are powerful and indispensable tools for imaging RNA in vivo and in vitro. To simultaneously visualize multiple RNA targets in a cell, it is necessary to develop probes which emit fluorescence with different colors by excitation at a single wavelength. We synthesized OMUpy1 and OMUpy2 in this study with a cyanine dye respectively conjugated at their 5' ends. A fluorescent analysis revealed these probes to have yellow or pink fluorescence derived from the cyanine dyes with an extremely large Stokes shift. Three color-coded fluorescent images were also obtained in the presence of target RNAs.

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