Optimization of Cold-Active Lipase Production from Psychrophilic Bacterium <i>Moritella</i> sp. 2-5-10-1 by Statistical Experimental Methods
-
- WANG Quanfu
- School of Marine and Technology, Harbin Institute of Technology
-
- ZHANG Chunyu
- School of Marine and Technology, Harbin Institute of Technology
-
- HOU Yanhua
- School of Marine and Technology, Harbin Institute of Technology
-
- LIN Xuezheng
- Key Lab of Marine Bioactive Substances, First Institute of Oceanography, State Oceanic, Administration
-
- SHEN Jihong
- Key Lab of Marine Bioactive Substances, First Institute of Oceanography, State Oceanic, Administration
-
- GUAN Xiangyu
- School of Ocean Sciences, China University of Geosciences
Bibliographic Information
- Other Title
-
- Optimization of Cold-Active Lipase Production from Psychrophilic Bacterium Moritella sp. 2-5-10-1 by Statistical Experimental Methods
Search this article
Abstract
Statistical experimental designs were applied to optimize cold-active lipase production by the psychrophilic bacterium Moritella sp. 2-5-10-1. First, a Plackett–Burmen design (PBD) was used to evaluate the significant effects of various fermentation parameters. The results indicated that soybean meal, temperature, and Tween-80 had significant influences on lipase production. The levels of these variables were optimized subsequently using central composite design (CCD). A quadratic regression model of cold-active lipase production was built, and verification experiments confirmed its validity. On subsequent scale-up in a 10-L bioreactor using optimized conditions, cold-active lipase production (30.56 U/mL) was obtained. The results clearly indicated that the model was adequate even on a large scale. To our knowledge, this is the first report of statistical optimization of cold-active lipase production by a psychrophilic bacterium.
Journal
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 77 (1), 17-21, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282681454830208
-
- NII Article ID
- 10031164686
-
- NII Book ID
- AA10824164
-
- COI
- 1:STN:280:DC%2BC3s3ntVSltA%3D%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL BIB ID
- 024226296
-
- PubMed
- 23291744
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed