Long-Chain Free Fatty Acid Profiling Analysis by Liquid Chromatography–Mass Spectrometry in Mouse Treated with Peroxisome Proliferator-Activated Receptor α Agonist

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  • TAKAHASHI Haruya
    Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
  • SUZUKI Hideyuki
    Kazusa DNA Research Institutes
  • SUDA Kunihiro
    Kazusa DNA Research Institutes
  • YAMAZAKI Yota
    Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
  • TAKINO Akihiro
    Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
  • KIM Young-Il
    Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
  • GOTO Tsuyoshi
    Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University Research Unit for Physiological Chemistry, Kyoto University
  • IIJIMA Yoko
    Kazusa DNA Research Institutes
  • AOKI Koh
    Kazusa DNA Research Institutes
  • SHIBATA Daisuke
    Kazusa DNA Research Institutes
  • TAKAHASHI Nobuyuki
    Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University Research Unit for Physiological Chemistry, Kyoto University
  • KAWADA Teruo
    Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University Research Unit for Physiological Chemistry, Kyoto University

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  • Long-chain free fatty acid profiling analysis by liquid chromatography-mass spectrometry in mouse treated with peroxisome proliferator-activated receptor alpha agonist

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Abstract

A change in the free fatty acid (FFA) profile reflects an alteration in the lipid metabolism of peripheral tissue. A high-throughput quantitative analysis method for individual FFAs therefore needs to be established. We report here an optimized LC-MS assay for a high-throughput and high-sensitivity analysis of the 10 major long-chain FFAs in mouse plasma and liver. This assay enables quantification of individual FFAs by using trace amounts of samples (2 µL of plasma and 10 mg of liver tissue). We apply this method to analyze the FFA profile of plasma and liver samples from an obese mouse model treated with bezafibrate, the peroxisome proliferator-activated receptor α (PPARα) agonist, and show a change in the FFA profile, particularly in the palmitoleic and oleic acid contents. This assay is useful for quantifying individual FFAs and helpful for monitoring the condition of lipid metabolism.

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