The Function of Snodprot in the Cerato-Platanin Family from Dactylellina cionopaga in Nematophagous Fungi
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- YU Hanying
- Department of Petroleum Engineering, Northeast Petroleum University Department of Petroleum Engineering, Northeast Petroleum University
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- DUAN Junxin
- Novozymes China Novozymes China
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- WANG Bin
- College of Life Sciences, University of Dundee College of Life Sciences, University of Dundee
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- JIANG Xianzhi
- Novozymes China Novozymes China
書誌事項
- タイトル別名
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- The Function of Snodprot in the Cerato-Platanin Family from <i>Dactylellina cionopaga</i> in Nematophagous Fungi
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Dactylellina cionopaga is a potential biocontrol agent of phytoparasitic nematodes. Here the functions of snodprot of D. cionopaga were analysed. The gene was transcribed with a higher level under inducing conditions with nematodes. The recombinant protein expressed in Pichia pastoris had a molecular weight of 14 kDa and might form polymers in its native state. In a concentration-dependent manner, snodprot changed the chemotaxis and increased the body-bend frequency of Caenorhabditis elegans, but did not induce immunity in the indicated plants significantly. The results of an immunofluorescence assay proved that snodprot was expressed during the development of traps and conidia. According to the parasitism mechanisms of nematophagous fungi and the chemotaxis and locomotion mechanisms of C. elegans, the possible active sites of snodprot were speculated to be ASE or ASI. The gene identification indicated that snodprot is a novel parasitism-related protein of nematophagous fungi, and possesses novel activity, different from other members of the cerato-platanin family.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 76 (10), 1835-1842, 2012
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681455063552
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- NII論文ID
- 130004053995
- 10031126467
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3s%2FjsFGntg%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 024035854
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- PubMed
- 23047092
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可