The AplⅠ Restriction-Modification System in an Edible Cyanobacterium, Arthrospira (Spirulina) platensis NIES-39, Recognizes the Nucleotide Sequence 5'-CTGCAG-3'

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  • The <i>Apl</i>I Restriction-Modification System in an Edible Cyanobacterium, <i>Arthrospira</i> (<i>Spirulina</i>) <i>platensis</i> NIES-39, Recognizes the Nucleotide Sequence 5′-CTGCAG-3′
  • The AplI restriction-modification system in an edible cyanobacterium, Arthrospira (Spirulina) platensis NIES-39, recognizes the nucleotide sequence 5′-CTGCAG-3′
  • AplI restriction-modification system in an edible cyanobacterium, Arthrospira (Spirulina) platensis NIES-39, recognizes the nucleotide sequence 5′-CTGCAG-3′

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The degradation of foreign DNAs by restriction enzymes in an edible cyanobacterium, Arthrospira platensis, is a potential barrier for gene-transfer experiments in this economically valuable organism. We overproduced in Escherichia coli the proteins involved in a putative restriction-modification system of A. platensis NIES-39. The protein produced from the putative type II restriction enzyme gene NIES39_K04640 exhibited an endonuclease activity that cleaved DNA within the sequence 5′-CTGCAG-3′ between the A at the fifth position and the G at the sixth position. We designated this enzyme AplI. The protein from the adjacent gene NIES39_K04650, which encodes a putative DNA (cytosine-5-)-methyltransferase, rendered DNA molecules resistant to AplI by modifying the C at the fourth position (but not the C at the first position) in the recognition sequence. This modification enzyme, M.AplI, should be useful for converting DNA molecules into AplI-resistant forms for use in gene-transfer experiments. A summary of restriction enzymes in various Arthrospira strains is also presented in this paper.

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