Properties and Physiological Functions of UDP-Sugar Pyrophosphorylase in<i>Arabidopsis</i>

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  • KOTAKE Toshihisa
    Division of Life Science, Graduate School of Science and Engineering, Saitama University
  • HOJO Sachiko
    Division of Life Science, Graduate School of Science and Engineering, Saitama University
  • YAMAGUCHI Daisuke
    Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University
  • AOHARA Tsutomu
    Division of Life Science, Graduate School of Science and Engineering, Saitama University
  • KONISHI Tomoyuki
    Division of Life Science, Graduate School of Science and Engineering, Saitama University
  • TSUMURAYA Yoichi
    Division of Life Science, Graduate School of Science and Engineering, Saitama University

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  • Properties and Physiological Functions of UDP-Sugar Pyrophosphorylase in Arabidopsis

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UDP-sugar pyrophosphorylase catalyzes the conversion of various monosaccharide 1-phosphates to the respective UDP-sugars in the salvage pathway. Using the genomic database, we cloned a putative gene for UDP-sugar pyrophosphorylase from Arabidopsis. Although relatively stronger expression was detected in the vascular tissue of leaves and the pollen, AtUSP is expressed in most cell types of Arabidopsis, indicating a housekeeping function in nucleotide sugar metabolism. Recombinant AtUSP expressed in Escherichia coli exhibited broad specificity toward monosaccharide 1-phosphates, resulting in the formation of various UDP-sugars such as UDP-glucose, -galactose, -glucuronic acid, -xylose and -L-arabinose. A loss-of-function mutation in the AtUSP gene caused by T-DNA insertion completely abolished male fertility. These results indicate that AtUSP functions as a UDP-sugar pyrophosphorylase in the salvage pathway, and that the generation of UDP-sugars from monosaccharide 1-phosphates catalyzed by AtUSP is essential for pollen development in Arabidopsis.

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