Transcriptional Regulation of cGMP-Dependent Protein Kinase II (cGK-II) in Chondrocytes

DOI Web Site Web Site PubMed 44 References Open Access
  • YUASA Keizo
    Department of Biological Science and Technology, The University of Tokushima Graduate School
  • UEHARA Shotaro
    Department of Biological Science and Technology, The University of Tokushima Graduate School
  • NAGAHAMA Masami
    Department of Biological Science and Technology, The University of Tokushima Graduate School
  • TSUJI Akihiko
    Department of Biological Science and Technology, The University of Tokushima Graduate School

Bibliographic Information

Other Title
  • Transcriptional regulation of cGMP-dependent protein kinase 2 (cGK-2) in chondrocytes

Search this article

Description

The C-type natriuretic peptide/natriuretic peptide receptor-B/cGMP pathway plays an important role in the regulation of endochondral ossification. In chondrocytes, the physiological effect of cGMP is mediated primarily by the activation of cGMP-dependent protein kinase II (cGK-II). In this study, we investigated the transcriptional regulation of cGK-II in chondrocytes. The expression pattern of cGK-II transcripts was examined during chondrogenic differentiation of ATDC5 cells. cGK-II mRNA was not detectable in undifferentiated cells, but increased dramatically prior to differentiation to the hypertrophic stage. To analyze the transcriptional regulation of cGK-II, the 5′-flanking region of the mouse cGK-II gene was isolated and characterized. The promoter activity of the cGK-II gene decreased markedly following deletion and mutagenesis of the putative Nkx-binding site between nucleotide positions −292 and −286. These results suggest that the homeobox gene Nkx family is critical for the transcriptional regulation of cGK-II during chondrogenesis.

Journal

References(44)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top