Purification and Biochemical Characterization of Recombinant Rice UDP-Arabinopyranose Mutase Generated in Insect Cells
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- KONISHI Tomoyuki
- Forestry and Forest Products Research Institute
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- MIYAZAKI Yasumasa
- Forestry and Forest Products Research Institute
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- YAMAKAWA Seiyei
- University of Tsukuba
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- IWAI Hiroaki
- University of Tsukuba
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- SATOH Shinobu
- University of Tsukuba
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- ISHII Tadashi
- Forestry and Forest Products Research Institute
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Abstract
Plants utilize UDP-arabinofuranose (UDP-Araf) in the biosynthesis of Araf-containing complex carbohydrates. UDP-Araf is synthesized from UDP-arabinopyranose by UDP-arabinopyranose mutases (UAMs). Here we describe the heterologous expression of rice (Oryza sativa) UAM genes in insect cells and report some of their enzymatic properties. Recombinant UAMs might serve as useful tools for the biosynthesis of UDP-Araf and might be better than chemical synthesis.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 74 (1), 191-194, 2010
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681455131392
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- NII Article ID
- 10027550650
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 10533150
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed